ELISpot Flex: Human IL-13 (HRP)

ELISpot Flex: Human IL-13 (HRP)

3471-2H

Publications: 32
Documents
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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT1318
Detection mAb25K2, biotin
Enzyme conjugateStreptavidin-HRP

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Delivery 4-9 business days

Shipping $0

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ELISpot substrate: TMB for HRP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-13 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Human IL-13 (HRP)
ApplicationELISpot
AnalyteIL-13
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-13.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
Human PBMC were incubated for 40 h in the absence or presence of PHA and the number of cells secreting IL-13 was analyzed by ELISpot.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IL-13

Analyte description

Interleukin 13 (IL-13) is produced by Th2-type cells, mast cells, and NK cells in response to activation. It regulates the secretion of IgE by B cells and the Th2-type cell development and suppresses inflammatory responses by regulation of macrophage function.

Alternative namesInterleukin 13, IL-13, IL13, P600
Cell typeT cell, Th2, Treg
Gene ID3596
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ELISpot Plus: Human IL-13 (HRP)